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The tender-clipped sequences that overhang the breakpoint are collapsed into a consensus sequence employing a various sequence alignment (MSA) supplied while in the seqAn library [27]. Wham applies three filters on the consensus sequences. Breakpoints will not be reported in conditions where consensus sequences are shorter than ten bp or have more
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Wham is suitable for paired-end Illumina libraries with typical insert sizes (~300bp-500bp). Wham integrates mate-pair mapping, split examine mapping, tender-clipping, substitute alignment and consensus sequence primarily based evidence to forecast SV breakpoints with single-nucleotide precision. Wham generates a put together pileup (catalog of rea